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KMID : 0881720220370010009
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Journal of Food Hygiene and Safety
2022 Volume.37 No. 1 p.9 ~ p.14
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Isolation and Identification of Fungi and Yeast Contaminated in Rice Cake (Garaetteok)
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Jo Ah-Hyeon
Kim Jung-Beom
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Abstract
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The purpose of this study was to analyze the hazard of fungi in Garaetteok (Korean rice cake) byisolating and identifying of fungi contaminated with Garaetteok and investigating the possibility of mycotoxin production.
Garaetteok used in this study were the ones that were returned back to the manufacturers in Jeollanam-dodue to the presence of foreign matters presumed to be fungi. The fungi foreign matter was collected and inoculatedon Potato dextrose agar, Malt extract agar, and Czapek yeast extract agar, and then cultured at 25oC for 7 days. Themicro-structure was observed under an optical microscope for the colonies in which pure isolation was confirmed. Thegene sequencing of the product of amplified PCR was analyzed using the ITS primer. Colony-1 and 2 maintained thesame properties in each tray, confirming that they were purely isolated. Budding cells were observed from the Colony-1, thus, it was determined to be yeast. Colony-2 was determined to be a fungus that belongs to Fusarium spp. as fusiformconidia were observed. As a result of gene sequencing, a total of 76 cases of fungi of Fusarium spp. were found,among which Fusarium solani was the most observed cases (53 cases). From the morphological and genetic identification,Colony-2 was identified as Fusarium spp., specifically, Fusarium solani. The fungi found in Fusarium spp.
produce mycotoxins such as nivalenol, zearalenone, and fumonisin, which may cause vomiting, diarrhea, and cancer.
Conclusively, the results confirm the possibility of mycotoxin production by Fusarium spp. isolated from Garaetteok.
Consequently, when an unknown fungus was found, it is necessary to isolate and identify the fungus, determinewhether it is a mycotoxin producing species, and strengthen relative administrative measures, accordingly.
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KEYWORD
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Fungi, Mycotoxin, Identification, Rice cake, Foreign matter
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